关键词: 天然产物化学实验, 科教融合, 盐酸小檗碱, 荧光增强, 活性氧

Abstract: Natural products chemistry experiment courses are vital practical part built upon theoretical foundations. However, these verification-focused experiments lack depth and hinder the development of students’ comprehensive abilities. To address this issue, we proposed integrating new scientific research findings into the design of comprehensive experiments, incorporating the concept of “double carbon”, and enhancing the depth of the course. In this experiment, we introduced cutting-edge research on photodynamic antimicrobial food packaging films with high fluorescence intensity into natural product chemistry experiments. Specifically, we incorporated berberine hydrochloride, a naturally occurring aggregation-induced emission agent, with phytic acid to create a salt that was added to the food packaging film, resulting in a photodynamic antimicrobial film with strong fluorescence. This experiment combined the fields of natural product chemistry, aggregation-induced emission, and food microbiology, and encouraged students to become familiar with the research process of theory-experiment-characterization-analysis. Through this integration, students could enrich their comprehensive knowledge, strengthen their practical skills, and foster their innovation consciousness, thus contributing to the cultivation of top talents in the field of forest chemical industry.

Key words: Natural product chemistry experiments, Integration of science and education, Berberine hydrochloride, Fluorescence enhancement, Reactive oxygen species