大学化学 >> 2024, Vol. 39 >> Issue (8): 247-254.doi: 10.3866/PKU.DXHX202312064

化学实验 上一篇    下一篇

基于交叉学科的本科生综合性实验设计——槲皮素对髓过氧化物酶氯化活性的影响研究

田蓉1,2, 杨亚迪1,2, 卢乃浩1,2   

  1. 1. 江西师范大学化学化工学院, 南昌 330022;
    2. 化学国家级实验教学示范中心(江西师范大学), 南昌 330022
  • 收稿日期:2023-12-19 修回日期:2024-02-20 发布日期:2024-03-11
  • 通讯作者: 田蓉 E-mail:tianrong@jxnu.edu.cn
  • 基金资助:
    国家自然科学基金(82260637;31960196)

Comprehensive Experimental Design of Undergraduate Students Based on Interdisciplinarity: Study on the Effect of Quercetin on Chlorination Activity of Myeloperoxidase

Rong Tian1,2, Yadi Yang1,2, Naihao Lu1,2   

  1. 1. College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, China;
    2. National Demonstration Center for Experimental Chemistry Education (Jiangxi Normal University), Nanchang 330022, China
  • Received:2023-12-19 Revised:2024-02-20 Published:2024-03-11
  • Contact: Rong Tian E-mail:tianrong@jxnu.edu.cn

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摘要： 介绍一个化学生物学交叉学科的综合实验。本实验紧密结合学科前沿，将科教深度融合。在体外生化反应体系中，运用化学和生物学的方法研究天然抗氧化物质槲皮素对生物体内髓过氧化物酶氧化还原循环的影响，通过分子对接方法和一系列的紫外-可见吸收光谱测试，阐明槲皮素影响髓过氧化物酶氯化活性的作用机制。该实验的实施有助于拓展学生视野、激发学生的科研兴趣和提高学生综合实验能力，为化学相关专业复合创新型人才培养提供有益的参考和借鉴。

关键词: 交叉学科, 槲皮素, 髓过氧化物酶, 氯化活性, 综合实验

Abstract: This paper presents a comprehensive experiment within the interdisciplinary field of chemical biology, closely aligned with the cutting-edge of the discipline, deeply integrating science and education. Utilizing both chemical and biological methods within an in vitro biochemical reaction system, this study investigates effects of the natural antioxidant quercetin on the redox cycle of myeloperoxidase (MPO) within biological organisms. The mechanism of quercetin affecting the chlorination activity of MPO is clarified through molecular docking techiniques and a series of UV-Vis absorption spectroscopy. The implementation of this experiment aids in broadening students’ perspectives, igniting their interest in scientific research, and enhancing their comprehensive experimental abilities. It offers valuable insights and examples for the development of multidisciplinary innovative talents in chemistry-related majors.

Key words: Interdisciplinary, Quercetin, Myeloperoxidase, Chlorination activity, Comprehensive experiment