大学化学 >> 2025, Vol. 40 >> Issue (2): 40-49.doi: 10.12461/PKU.DXHX202405121

所属专题： 教学型仪器的研制&改进与实验教学

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自制表面解吸大气压化学电离质谱装置检测不饱和脂肪酸

张小平, 胡平花, 张军, 杨文雯, 曹晶晶   

  1. 东华理工大学, 江西省质谱科学与仪器重点实验室, 南昌 330013
  • 收稿日期:2024-05-17 录用日期:2024-10-10 发布日期:2025-02-22
  • 通讯作者: 张小平 E-mail:zhangxpsunshine@163.com
  • 基金资助:
    江西省高等学校教学改革研究项目(JXJG-21-6-23)；江西国家级大学生创新创业训练计划(202310405004X)；东华理工大学实验技术开发项目(DHSY-202315)；东华理工大学校级教改项目(1310101295)

Self-Developed Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry for Detection of Unsaturated Fatty Acids

Xiaoping Zhang, Pinghua Hu, Jun Zhang, Wenwen Yang, Jingjing Cao   

  1. Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology, Nanchang 330013, China
  • Received:2024-05-17 Accepted:2024-10-10 Published:2025-02-22
  • Contact: Xiaoping Zhang E-mail:zhangxpsunshine@163.com

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摘要： 为应对当前化学教学实验多以验证性实验为主且学生自主创新能力不足的现状，设计了一项新实验——“自制表面解吸大气压化学电离质谱装置检测不饱和脂肪酸”。通过让学生自主搭建常压电晕放电电离装置，他们可以深入理解电离与环氧化的原理，激发实验主动性。这种自制装置简便高效，大幅降低教学成本，同时避免使用有机试剂，体现绿色环保理念。在本实验中，学生将研究新型活性物种水自由基阳离子(H2O)2+·与油酸的C＝C键的环氧化反应，以实现对植物油中油酸的快速定量分析。该实验考察了分析化学的基本操作及有机质谱实验技能，旨在培养学生的动手能力及科学创新思维，非常适合用于本科化学实验教学。

关键词: 油酸, 直接质谱分析, 定量分析, 教改实验, 无需样品预处理

Abstract: In response to the current situation in chemistry education, where experiments predominantly focus on verification and students’ ability for independent innovation is limited, a novel experiment titled “Self-developed Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry for Detection of Unsaturated Fatty Acids” has been designed. By guiding students to build their own atmospheric corona discharge ionization device, the experiment offers a deep understanding of the principles of ionization and epoxidation, encouraging experimental initiative. The homemade device is simple, efficient, and significantly reduces teaching costs, while also avoiding the use of organic reagents, reflecting an environmentally friendly approach. In this experiment, students investigate the epoxidation of the C＝C bond of oleic acid with the water radical cation (H2O)2+·, a novel active species, for the rapid quantitative analysis of oleic acid in vegetable oils. The experiment emphasizes essential analytical chemistry techniques and organic mass spectrometry skills, with the goal of cultivating students’ hands-on abilities and fostering scientific innovation. This experiment is particularly suitable for undergraduate chemistry laboratory courses.

Key words: Oleic acid, Direct mass spectrometry, Quantitative analysis, Teaching reform experiment, No sample pretreatment