大学化学 >> 2025, Vol. 40 >> Issue (4): 390-398.doi: 10.12461/PKU.DXHX202405147

化学实验 上一篇    下一篇

荧光定量分析实验的改进——化学计量学辅助的三维荧光法同时测定食品中的罗丹明6G和罗丹明123

文密, 贾宝硕, 柴永琪, 王童, 刘剑波, 吴海龙   

  1. 湖南大学化学化工学院, 化学生物传感与计量学国家重点实验室, 长沙 410082
  • 收稿日期:2024-05-22 录用日期:2024-09-18 发布日期:2025-04-25
  • 通讯作者: 王童, 刘剑波 E-mail:wangtong@hnu.edu.cn;liujianbo@hnu.edu.cn
  • 基金资助:
    2024年湖南省普通本科高校教学改革研究项目(202401000432)；国家自然科学基金(22204049)；湖南省教育厅科学研究重点项目(23A0028)

Improvement of Fluorescence Quantitative Analysis Experiment: Simultaneous Determination of Rhodamine 6G and Rhodamine 123 in Food Using Chemometrics-Assisted Three-Dimensional Fluorescence Method

Mi Wen, Baoshuo Jia, Yongqi Chai, Tong Wang, Jianbo Liu, Hailong Wu   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
  • Received:2024-05-22 Accepted:2024-09-18 Published:2025-04-25
  • Contact: Tong Wang, Jianbo Liu E-mail:wangtong@hnu.edu.cn;liujianbo@hnu.edu.cn

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摘要： 仪器分析实验“分子荧光法测定罗丹明B的含量”存在实验过于简单、未考虑实际情况等问题。因此，本改进实验在三维荧光扫描模式下获取样本数据，不进行复杂预处理，而是运用化学计量学算法解析出目标分析物的纯信号，进而实现了染色辣椒中罗丹明6G和123的同时测定。本改进实验提高了学生全面考虑问题和创新解决问题的能力。

关键词: 罗丹明6G, 罗丹明123, 三维荧光, 化学计量学, 染色辣椒

Abstract: The existing instrument analysis experiment, “Molecular fluorescence method for the determination of rhodamine B”, is overly simplistic and dose not account for real-world complexities. To address these issues, this improved experiment utilizes three-dimensional fluorescence scanning to acquire sample data. Instead of complex preprocessing steps, chemometric algorithms are applied to extract the pure signals of the target analytes, enabling the simultaneous determination of rhodamine 6G and rhodamine 123 in dyed chili. This improvement enhances students' ability to approach problems comprehensively and develop innovative solutions.

Key words: Rhodamine 6G, Rhodamine 123, Three-dimensional fluorescence method, Chemometrics, Dyed chili