大学化学 >> 2026, Vol. 41 >> Issue (10): 316-325.doi: 10.12461/PKU.DXHX202510041

化学实验 上一篇    

科教融合导向的化学综合实验设计——以铜离子特异性荧光探针的研发与应用为例

谭梁玲, 张崇华, 刘贤军, 徐逸婷, 刘万强   

  1. 湖南科技大学化学化工学院, 湖南 湘潭 411100
  • 收稿日期:2025-10-15 录用日期:2025-11-24 发布日期:2026-10-13
  • 通讯作者: 张崇华 E-mail:chonghua1226@hnu.edu.cn Chonghua Zhang
  • 基金资助:
    湖南省学位与研究生教学改革研究项目(2022JGSZ054)；湖南省教育厅项目教研教改项目(HNJG-20230658)；2024年湖南省学位与研究生教学改革研究项目(2024JGYB198)

Design of a research-education integrated comprehensive chemistry experiment: a case study on the development and application of a copper-specific fluorescent probe

Liangling Tan, Chonghua Zhang, Xianjun Liu, Yiting Xu, Wanqiang Liu   

  1. College of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411100, Hunan Province, China
  • Received:2025-10-15 Accepted:2025-11-24 Published:2026-10-13
  • Contact: Chonghua Zhang E-mail:chonghua1226@hnu.edu.cn

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摘要： 科教融合是培养学生创新能力、激发学生学习兴趣的重要途径。本文结合本实验室的科研成果，设计了一项以科教融合为导向的化学综合实验。即通过设计合成基于2-吡啶甲酸酯的反应型荧光探针THL-Cu，并借助探针的荧光及颜色变化进行铜离子的含量鉴定。该探针对铜离子表现出高灵敏度，检出限为0.2 μmol·L-1，并具有特异性的荧光增强响应，增强倍数约15倍。通过将探针合成、标准曲线绘制、荧光光谱测定、铜离子含量分析、铜离子回收等过程结合，学生可掌握有机合成、仪器分析、数据处理等专业知识，并融合绿色化学理念。本实验通过科教融合，实现了知识、能力与思政教育的协同培养。

关键词: 荧光探针, 铜离子检测, 综合化学实验, 科教融合, 课程思政

Abstract: The integration of scientific research and education serves as a crucial approach for cultivating students’ innovative abilities and stimulating their learning interest. This study presents a research-education integrated comprehensive chemistry experiment based on our laboratory’s scientific achievements. The experiment involves the design and synthesis of THL-Cu, a reaction-based fluorescent probe featuring a 2-pyridylcarboxylate recognition unit for selective Cu2+ detection through fluorescence and colorimetric responses. The probe demonstrates a detection limit of 0.2 μmol·L-1 for Cu2+ and exhibits a remarkable 15-fold fluorescence enhancement exclusively for copper ions. By combining probe synthesis, standard curve plotting, fluorescence spectroscopy, copper content analysis, and ion recovery processes, students gain comprehensive training in organic synthesis, instrumental analysis, and data processing while incorporating green chemistry principles. This experiment successfully achieves the coordinated cultivation of knowledge acquisition, technical skills, and ideological education through research-teaching integration.

Key words: Fluorescent probe, Copper(II) detection, Comprehensive chemistry experiment, Research-education integration, Curriculum-based ideological and political education