大学化学 >> 2026, Vol. 41 >> Issue (10): 216-225.doi: 10.12461/PKU.DXHX202511032

科普 上一篇    

明察秋毫的“显色反应”——快速检测牛奶细菌污染的科普实验

马俊林, 董阿力德尔图, 吴海霞   

  1. 内蒙古大学化学化工学院, 内蒙古 呼和浩特 010021
  • 收稿日期:2025-11-05 录用日期:2026-01-07 发布日期:2026-10-13
  • 通讯作者: 董阿力德尔图, 吴海霞 E-mail:dongali@imu.edu.cn;chmwuhx@imu.edu.cn Alideertu Dong, Haixia Wu
  • 基金资助:
    内蒙古自治区高等学校创新团队发展计划(NMGIRT2210)；内蒙古自治区研究生教育教学改革研究项目(JGCG2023018)

Eagle-eye “color reaction”: A science popularization experiment for rapid detection of bacterial contamination in milk

Junlin Ma, Alideertu Dong, Haixia Wu   

  1. College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, Inner Mongolia Autonomous Region, China
  • Received:2025-11-05 Accepted:2026-01-07 Published:2026-10-13
  • Contact: Alideertu Dong, Haixia Wu E-mail:dongali@imu.edu.cn;chmwuhx@imu.edu.cn

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摘要： 针对食品安全关注度的日益提升，本文聚焦牛奶细菌污染问题，结合刃天青强氧化特性与高灵敏荧光技术，实现了牛奶高浓度细菌污染的可视化比色检测与低浓度细菌污染的荧光检测，并拓展检测维生素C含量。同时，通过层次化科普实践，让不同人群了解化学检测、牛奶相关知识，感受食品安全保障中化学的重要作用与独特魅力。

关键词: 氧化还原反应, 细菌检测, 刃天青, 维生素C, 科普实践

Abstract: In response to growing public concerns regarding food safety, this study addresses the issue of bacterial contamination in milk. Utilizing the strong oxidative characteristics of resazurin combined with highly sensitive fluorescence technology, we achieved visual colorimetric detection of high-concentration bacterial contamination and fluorescence-based detection of low-concentration bacterial contamination in milk. The methodology was further extended to quantify vitamin C content. Through tiered science popularization initiatives, this work enables diverse audiences to comprehend chemical detection principles and milk-related knowledge while demonstrating chemistry’s crucial role and unique value in food safety assurance.

Key words: Redox reaction, Bacteria detection, Resazurin, Vitamin C, Science popularization practice