大学化学 >> 2017, Vol. 32 >> Issue (1): 58-61.doi: 10.3866/PKU.DXHX201609031

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催化荧光法测定痕量铜的探究性实验设计与实践

盛丽*(),赵志惠,米莹,苏碧泉,荆王莉,陶彩虹   

  • 发布日期:2017-01-13
  • 通讯作者: 盛丽 E-mail:shengl@mail.lzjtu.cn
  • 基金资助:
    兰州交通大学实验教改项目(201609);甘肃省财政厅2014年高校基本科研业务费项目(213059)

Measurement of Tracing Copper Using Catalytic Kinetic Fluorescence Method: Design and Practice of an Inquiry Experiment

Li SHENG*(),Zhi-Hui ZHAO,Ying MI,Bi-Quan SU,Wang-Li JING,Cai-Hong TAO   

  • Published:2017-01-13
  • Contact: Li SHENG E-mail:shengl@mail.lzjtu.cn
  • Supported by:
    兰州交通大学实验教改项目(201609);甘肃省财政厅2014年高校基本科研业务费项目(213059)

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摘要：

基于铜（Ⅱ）对高碘酸钾氧化番红花红T具有催化作用，建立催化动力学荧光法测定痕量铜的新体系。体系在加入铜（Ⅱ）后，在580 nm波长处的荧光强度明显降低，且在2.50-32.50 ng·mL-1范围内铜（Ⅱ）的浓度与体系的lg（F0/F）呈线性关系。基于催化动力学荧光分析法测定痕量铜的新方法实验，探讨了仪器分析实验课探究性实验的设计与实施方法。同时，为实现探究性实验教学，教师要严把命题关和可行性分析关，以期达到探究性实验设计的教学效果。

关键词: 仪器分析实验, 探究性实验设计, 铜, 催化动力学荧光法

Abstract:

Based on the catalytic effects of Cu(Ⅱ) on KIO4 oxidized safranineT, a newsystemfor determining tracing copper with catalytic kinetic fluorescencemethod is established.After adding Cu(Ⅱ), the fluorescence intensity at 580 nmis significantly reduced.Within the concentration range of 2.50-32.50 ng·mL-1, a linear relationship between the concentration of Cu(Ⅱ) and lg(F0/F) in the system is observed. Based on this new experiment, the design and implementation of instrumental analysis laboratory is investigated. Meanwhile, in order to achieve high quality of the inquiry experiment teaching, teachers should be responsible for proposition and feasibility.

Key words: Instrumental analysis laboratory, Design of inquiry experiment, Copper, Catalytic kinetic fluorescence method