大学化学 >> 2024, Vol. 39 >> Issue (4): 81-86.doi: 10.3866/PKU.DXHX202311013

所属专题： 化学实验教学和管理(2023)

专题 上一篇    下一篇

基于阀切换UHPLC-IC联用技术的实验教学探索

毛侦军, 顾昊睿, 车海燕, 林旭锋   

  1. 浙江大学化学系, 国家级实验教学示范中心, 杭州 310058
  • 收稿日期:2023-11-01 修回日期:2023-12-18 发布日期:2023-12-29
  • 通讯作者: 毛侦军 E-mail:maozhenjun@zju.edu.cn
  • 基金资助:
    浙江省基础公益研究计划资助项目(LTGC24B050003)；浙江大学线上线下混合式课程培育项目；浙江大学一流课程建设项目

Exploration on Experiment Teaching of UHPLC-IC Based on Valve Switching Method

Zhenjun Mao, Haorui Gu, Haiyan Che, Xufeng Lin   

  1. National Demonstration Center for Experimental Education, Chemistry Department, Zhejiang University, Hangzhou 310058, China
  • Received:2023-11-01 Revised:2023-12-18 Published:2023-12-29

PDF

190

赞

摘要： 具有阀切换的超高效液相色谱-离子色谱联用(UHPLC-IC)技术适用于同时分析含有机物和无机阴离子的样品，该联用技术简化了前处理的过程，消除了复杂基体，对无机阴离子进行富集，实现有机物和无机阴离子的同时分析，提高了色谱分析效率。将阀切换UHPLC-IC联用技术引入到本科生仪器分析实验课堂中，加深了学生对新型分析技术独特优势的理解，提高了本科生解决复杂实际问题的能力，拓宽和提升了仪器分析实验的教学内涵，有利于创新性和综合性人才的培养。

关键词: 阀切换, 超高效液相色谱, 离子色谱, 仪器分析实验

Abstract: The technology of ultrahigh performance liquid chromatography (UHPLC) and ion chromatography (IC) with valve switching method is suitable for the simultaneous analysis of samples containing organic compounds and inorganic anions, which simplifies the pretreatment process, eliminates the complex matrix, enriches inorganic anions, realizes the simultaneous analysis of organic compounds and inorganic anions, and improves the efficiency of chromatographic analysis. The technology of ultrahigh performance liquid chromatography and ion chromatography with valve switching method is introduced into the undergraduate instrument analysis experiment class, which deepens the students’ understanding of the special advantages of the new technology, improves the undergraduates’ ability to solve complex practical problems, widens the field and enriches the content of instrument analysis experiment. Of course, it is conducive to the cultivation of innovative and comprehensive talents.

Key words: Valve switching, UHPLC, IC, Instrumental analysis experiment