大学化学 >> 2024, Vol. 39 >> Issue (6): 271-277.doi: 10.3866/PKU.DXHX202309062

所属专题： 第4届全国大学生化学实验创新设计大赛

化学实验 上一篇    下一篇

基于纸芯片的大肠杆菌检测的创新实验教学

涂琴1, 陶安驹1, 马彤彤1,2, 王进义1   

  1. 1 西北农林科技大学化学与药学院, 陕西 杨凌 712100;
    2 楚雄师范学院, 云南 楚雄 675099
  • 收稿日期:2023-09-18 修回日期:2024-01-10 发布日期:2024-06-07
  • 通讯作者: 涂琴, 王进义 E-mail:tuqin@nwsuaf.edu.cn;jywang@nwsuaf.edu.cn
  • 基金资助:
    国家自然科学基金(21505105)

Innovative Experimental Teaching of Escherichia coli Detection Based on Paper Chip

Qin Tu1, Anju Tao1, Tongtong Ma1,2, Jinyi Wang1   

  1. 1 College of Chemistry & Pharmacy, Northwest A & F University, Yangling 712100, Shaanxi Province, China;
    2 Chuxiong Normal University, Chuxiong 675099, Yunnan Province, China
  • Received:2023-09-18 Revised:2024-01-10 Published:2024-06-07
  • Contact: Qin Tu, Jinyi Wang E-mail:tuqin@nwsuaf.edu.cn;jywang@nwsuaf.edu.cn

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摘要： 设计了一种基于微流控纸基芯片用于特异性检测E. coli的综合创新实验。制备纸基芯片，在芯片上实现水样中E. coli的检测。主要内容包括纸芯片的制备，纸芯片上E. coli的裂解和检测，检测条件的优化，以及实际样品中E. coli的检测。该实验结合学科前沿，综合性和创新性较强。可实现“基础性实验→综合性实验→科研性实验”为特色的实践育人理念，提高学生分析和解决问题的能力。

关键词: 大肠杆菌, 纸基芯片, 可视化定量检测, 创新实验

Abstract: An innovative experimental approach based on a microfluidic paper-based chip has been designed for specific detection of Escherichia coli (E. coli). The chip is fabricated for detecting E. coli in water samples. The main contents include the preparation of paper chips, lysis of E. coli on the chip, detection of E. coli, optimization of detection conditions, and detection of E. coli in actual samples. This experiment integrates cutting-edge disciplines, demonstrating robust comprehensive and innovative characteristics. It aligns with the practical education concept marked by the progression from “basic experiment → comprehensive experiment → research-oriented experiment”, effectively strengthening students’ capacities for problem analysis and solution.

Key words: E. coli, Paper-based chip, Visual and quantitative detection, Innovative experimental