新冠病毒光热/比色双模态定量综合性实验设

doi:10.12461/PKU.DXHX202402027

大学化学 >> 2025, Vol. 40 >> Issue (2): 1-10.doi: 10.12461/PKU.DXHX202402027

所属专题：教学型仪器的研制&改进与实验教学

新冠病毒光热/比色双模态定量综合性实验设

曾景斌¹, 王文静¹, 赵田雨¹, 郭庚辰¹, 矫春鹏¹, 江永忠², 温聪颖¹

1 中国石油大学(华东)化学化工学院, 山东青岛 266580;
2 湖北省疾病预防控制中心, 武汉 430065

收稿日期:2024-02-08 录用日期:2024-04-15 发布日期:2025-02-22
通讯作者: 温聪颖 E-mail:flcyxt@163.com
基金资助:
国家自然科学基金面上项目(21876206,22376218)；山东省自然科学基金面上项目(ZR2020MB064)；山东省重大基础研究项目(ZR2020ZD13)；中国石油大学教学改革项目(CM2022027,CZ2022009)

Design of a Comprehensive Experiment for Photothermal/Colorimetric Dual-Mode Quantification of SARS-CoV-2

Jingbin Zeng¹, Wenjing Wang¹, Tianyu Zhao¹, Gengchen Guo¹, Chunpeng Jiao¹, Yongzhong Jiang², Congying Wen¹

1 College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, Shandong Province, China;
2 Hubei Provincial Center for Disease Control and Prevention, Wuhan 430065, China

Received:2024-02-08 Accepted:2024-04-15 Published:2025-02-22
Contact: Congying Wen E-mail:flcyxt@163.com

摘要/Abstract

摘要： 设计了“新冠病毒光热/比色双模态定量综合性实验”，通过开发在近红外光下具有高效光热转换效率的标记纳米探针，结合自行搭建的小型光热成像仪，有效克服了商用胶体金试纸条灵敏度低和不能准确定量的问题。该法操作简便快速，只需15 min即可完成，能够实现裸眼比色定性和光热信号定量检测，检测限低至1.2 pg·mL^-1，相比于胶体金试纸条，不仅实现了准确定量，且灵敏度提高了近10000倍，极大地缩短了病毒检测的窗口期。教学实践表明，该实验能够提高学生的综合能力、开拓其学术视野，并培养学生的科研素养和创新潜能。

关键词: Au_shell-Fe₃O₄纳米粒子, 比色检测, 光热检测, 试纸条, 新冠病毒

Abstract: We have designed a comprehensive experiment for the photothermal/colorimetric dual-mode quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). By developing label nanoprobes with high photothermal conversion efficiency under near-infrared light and integrating them with a self-built small photothermal imager, we have effectively addressed the limitations of commercial colloidal gold test strips, such as low sensitivity and inability to quantify. This method is rapid and user-friendly, requiring only 15 minutes to complete, and enables both naked-eye colorimetric qualification and photothermal accurate quantification. With a detection limit as low as 1.2 pg·mL^-1, this method surpasses commercial colloidal gold test strips by achieving accurate quantitative detection and improving sensitivity by nearly 10,000 times, significantly reducing the detection window period. Teaching practices have shown that this experiment enhances students’ comprehensive abilities, expands their academic horizons, and nurtures their scientific research literacy and innovation potential.

Key words: Au_shell-Fe₃O₄ nanoparticles, Colorimetric detection, Photothermal detection, Test strip, SARS-CoV-2

曾景斌, 王文静, 赵田雨, 郭庚辰, 矫春鹏, 江永忠, 温聪颖. 新冠病毒光热/比色双模态定量综合性实验设[J]. 大学化学, 2025, 40(2): 1-10.

Jingbin Zeng, Wenjing Wang, Tianyu Zhao, Gengchen Guo, Chunpeng Jiao, Yongzhong Jiang, Congying Wen. Design of a Comprehensive Experiment for Photothermal/Colorimetric Dual-Mode Quantification of SARS-CoV-2[J]. University Chemistry, 2025, 40(2): 1-10.

链接本文: https://www.dxhx.pku.edu.cn/CN/10.12461/PKU.DXHX202402027

https://www.dxhx.pku.edu.cn/CN/Y2025/V40/I2/1

参考文献

[1] Barr, C. A.; Brodeur, D. R.; Kumar, U.; Heilman, D. W. J. Chem. Educ. 2022, 99, 3899.
[2] 贾琼, 马玖彤, 许海. 化学教育(中英文), 2021, 42 (24), 54.
[3] 温聪颖, 訾敏, 张瑞巧, 赵令今. 实验室研究与探索, 2021, 40 (6), 11.
[4] Mutambuki, J. M.; Fynewever, H.; Douglass, K.; Cobern, W. W.; Obare, S. O. J. Chem. Educ. 2019, 96, 1591.
[5] 邱玉婷, 史成坤, 齐海涛, 赵立冬. 实验技术与管理, 2021, 38 (4), 250.
[6] Morens, D. M.; Fauci, A. C. Cell 2020, 182, 1077.
[7] Coronavirus Disease (COVID-19) Situation Dashboard. [2024-03-03]. https://covid19.who.int/
[8] Bahadır, E. B.; Sezgintürk, M. K. TrAC-Trend. Anal. Chem. 2016, 82, 286.
[9] Wu, K.; Green, A. A. Nat. Biomed. Eng. 2022, 6, 928.
[10] Kim, K.; Lee, B. B.; Park, J. H.; Lee, K. J.; Kwak, T. J.; Son, T.; Shin, Y. B.; Im, H.; Kim, M. J. Mater. Horiz. 2023, 10, 1697.
[11] Huang, X.; Aguilar, Z. P.; Xu, H.; Lai, W.; Xiong, Y. Biosens. Bioelectron. 2016, 75, 166.
[12] Daniel, Q. J.; Arben, M. Biosens. Bioelectron. 2015, 73, 47.
[13] Claudio, P.; Amadeo, S. T.; José, F. B.; Enric, C.; Celia, F. C.; Hu, L. M.; Lourdes, R.; Ruslan, Á. D.; Emily, P. N.; Stefano, C.; et al. Nat. Protoc. 2020, 15, 3788.
[14] Geertruida, A. P. T.; Jan, H. W.; Marjo, K.; Luciënne, B. J. M. B.; Aart, V. A. Anal. Chim. Acta. 2012, 402, 593.
[15] Taranova, N. A.; Berlina, A. N.; Zherdev, A. V.; Dzantiev, B. B. Biosens. Bioelectron. 2015, 63, 255.
[16] Hu, J.; Jiang, Y. Z.; Tang, M.; Wu, L. L.; Xie, H. Y.; Zhang, Z. L.; Pang, D. W. Anal. Chem. 2019, 91, 1178.
[17] Liang, P.; Guo, Q.; Zhao, T.; Wen, C. Y.; Tian, Z.; Shang, Y.; Xing, J.; Jiang, Y.; Zeng, J. Anal. Chem. 2022, 94, 8466.
[18] Li, J.; Liang, P.; Zhao, T.; Guo, G.; Zhu, J.; Wen, C.; Zeng, J. Anal. Bioanal. Chem. 2023, 415, 545.
[19] Zhao, T.; Liang, P.; Ren, J.; Zhu, J.; Yang, X.; Bian, H.; Li, J.; Cui, X.; Fu, C.; Xing, J.; et al. Anal. Chim. Acta. 2023, 1255, 341102.
[20] 曾泳淮, 林树昌. 分析化学(仪器分析部分). 第2版. 北京: 高等教育出版社, 2004: 76-77.

新冠病毒光热/比色双模态定量综合性实验设

Design of a Comprehensive Experiment for Photothermal/Colorimetric Dual-Mode Quantification of SARS-CoV-2

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

Metrics

推荐阅读 0

[1]	方琪珑, 李懿绮, 生江伊徽, 袁荃, 谈洁. 神奇的农残检测试纸条——用于有机磷农药残留检测的核酸适配体侧流层析试纸条[J]. 大学化学, 2024, 39(5): 80 -89 .
[2]	杨迪, 魏嘉怡, 翟红, 王昕, 孙太明, 宋浩乐, 王海雁. 神奇“魔法条”之新冠病毒的快速检测[J]. 大学化学, 2024, 39(4): 373 -381 .
[3]	钱嘉梁, 罗崇悦, 陈建成. 光能使者——耀光灭“疾”[J]. 大学化学, 2022, 37(9): 2202027 - .
[4]	高耿欣, 陈建成. 病毒哪里藏——核酸检测金标准[J]. 大学化学, 2022, 37(9): 2205031 - .
[5]	王荷芳. 疫情下的仪器分析课程在线教学设计与探索——以绪论为例[J]. 大学化学, 2020, 35(5): 75 -80 .
[6]	李天荣, 苏军霞, 陈凤娟, 李华, 海军, 梁永民, 汪宝堆. 碳点的合成及对锰离子的比色检测——推荐一个大学化学综合实验[J]. 大学化学, 2020, 35(12): 206 -211 .