大学化学 >> 2024, Vol. 39 >> Issue (5): 80-89.doi: 10.3866/PKU.DXHX202310004

所属专题： 第4届全国大学生化学实验创新设计大赛

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神奇的农残检测试纸条——用于有机磷农药残留检测的核酸适配体侧流层析试纸条

方琪珑, 李懿绮, 生江伊徽, 袁荃, 谈洁   

  1. 湖南大学化学化工学院, 长沙 410082
  • 收稿日期:2023-10-10 修回日期:2024-02-02 发布日期:2024-03-01
  • 通讯作者: 袁荃, 谈洁 E-mail:yuanquan@whu.edu.cn;tanjie0416@hnu.edu.cn

Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection

Qilong Fang, Yiqi Li, Jiangyihui Sheng, Quan Yuan, Jie Tan   

  1. College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
  • Received:2023-10-10 Revised:2024-02-02 Published:2024-03-01
  • Contact: Quan Yuan, Jie Tan E-mail:yuanquan@whu.edu.cn;tanjie0416@hnu.edu.cn

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摘要： 有机磷农药是农业生产中使用最广泛的农药之一，其大量使用造成了农作物表面、水体和土壤等环境中的农药残留，严重破坏生态环境，威胁人体健康。因此，快速、准确地检测环境中的有机磷农药残留对于控制农药使用和污染具有重要意义。传统色谱、光谱检测方法依赖于大型仪器和专业的技术人员，存在检测成本高、耗时长等缺点。因此，本文创新性地制备了一种基于核酸适配体的胶体金侧流层析试纸条，用于果蔬中有机磷农药残留的快速和准确检测。由于该试纸条具有灵敏、便携、易于操作的优点，能现场应用在实际的蔬菜样品检测中，具有很好的科普性和可推广性。另外，本科普实验操作过程逻辑清晰、内容详实，并且针对不同类别的人群，设计多样化的实验过程和实验方案，能够很好地激发大家对化学的兴趣和提升对食品安全的意识。

关键词: 胶体金, 侧流层析试纸条, 核酸适配体, 有机磷农药, 科普

Abstract: Organophosphorus pesticides rank among the most extensively used pesticides in agriculture, leading to widespread contamination of water bodies, soil, and vegetation, thereby disrupting ecosystems and posing health risks. Consequently, rapid and precise detection of organophosphorus pesticide residues is crucial for effective pesticide management. Conventional analytical methods such as chromatography and spectroscopy rely on sophisticated equipment and specialized personnel, incurring high costs and time consumption. In response, this study presents an innovative approach involving the development of aptamer-based colloidal gold lateral flow test strips for the swift and accurate detection of residual organophosphorus pesticides in fruits and vegetables. These test strips offer advantages such as sensitivity, portability, and ease of operation, enabling on-site application in real sample detection and demonstrating excellent applicability. Furthermore, the logical operation process of these test strips facilitates science popularization and education. Diverse experimental processes and schemes tailored for different audiences serve to stimulate interest in chemistry and science while enhancing awareness of food safety.

Key words: Colloidal gold, Lateral flow test strip, Aptamers, Organophosphorus pesticide, Popularization of science