大学化学 >> 2026, Vol. 41 >> Issue (8): 276-285.doi: 10.12461/PKU.DXHX202507111

科普 上一篇    下一篇

玩“转”芯片，让DNA看得见

唐艾佳, 李欣泽, 石雅宁, 方雪恩   

  1. 复旦大学化学系, 上海 200438
  • 收稿日期:2025-07-28 录用日期:2025-09-25 发布日期:2026-08-25
  • 通讯作者: 方雪恩 E-mail:fxech@fudan.edu.cn Xueen Fang
  • 基金资助:
    复旦大学本科教学内涵提升计划及本科教学研究与改革实践项目(FD2023A223126)；国家自然科学基金((22374029)；上海市科技创新行动计划(23ZR1403200)；国家高层次人才计划(QWH1615018)

Rotating microfluidics to visualize DNA

Aijia Tang, Xinze Li, Yaning Shi, Xueen Fang   

  1. Department of Chemistry, Fudan University, Shanghai 200438, China
  • Received:2025-07-28 Accepted:2025-09-25 Published:2026-08-25
  • Contact: Xueen Fang E-mail:fxech@fudan.edu.cn

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摘要： 基于旋转式微流控芯片技术，设计了一套操作简便、结果可视化的DNA分析实验，旨在实现让DNA“看得到、看得准、看得快”。以转基因大豆快速鉴定为例，提取非转基因和两种转基因(BPS-CV127-9、MON87701)大豆粉末的DNA，结合紫外分光光度法和荧光法进行初步鉴定。通过多色荧光鉴定DNA的方法流程简易且趣味性强，适合中小学生及学龄前儿童动手参与；构建芯片离心机、恒温驱动微流控芯片化学反应，利用沉淀法、pH指示剂法、荧光指示法观测结果，实现快速特异性DNA鉴定，适用于高中生、大学生开展科普活动和综合实验。此外，基于此实验可面向大众辟谣不科学的转基因作物鉴别方法，并科普相关科学原理与国家政策。本实验以多种有趣的形式开展梯度科普，涵盖的受众群体广泛，以期让公众体会到化学的重要性和化学之美。

关键词: 微流控, DNA, 梯度科普, 转基因, 快速检测

Abstract: This study presents a user-friendly and visually intuitive DNA analysis experiment utilizing rotary microfluidic chip technology, designed to achieve “visible, accurate, and rapid” DNA detection. Using transgenic soybean identification as a model system, we extracted DNA from non-transgenic and two transgenic soybean varieties (BPS-CV127-9 and MON87701), followed by preliminary characterization via UV spectrophotometry and fluorescence analysis. The multicolor fluorescence-based DNA detection method features straightforward procedures and high engagement value, making it suitable for K-12 students and even preschool participants. By employing manual centrifugation and heating plates instead of conventional instruments to drive microfluidic chip reactions, we enabled result visualization through precipitation, pH indicators, and fluorescent markers. This approach is particularly suitable for high school and undergraduate laboratory activities. Furthermore, this experiment serves as an effective platform to debunk pseudoscientific claims about genetically modified crop identification while promoting evidence-based scientific literacy and national regulatory policies. Through tiered outreach activities, we aim to demonstrate chemistry's societal relevance and intrinsic beauty to diverse audiences.

Key words: Microfluidics, DNA, Tiered science outreach, Genetically modified organisms (GMOs), Rapid detection