大学化学 >> 2026, Vol. 41 >> Issue (6): 175-184.doi: 10.12461/PKU.DXHX202511193

专题 上一篇    

化学“101计划”微流控分析理论与实践教学探索

刘林1, 辜敏1, 严仲毅1, 熊桧文2, 方雪恩1   

  1. 1 复旦大学化学系, 上海 200433;
    2 上海市儿童医院, 上海交通大学医学院附属儿童医院检验科, 上海 200011
  • 收稿日期:2025-11-26 录用日期:2026-02-06 发布日期:2026-06-18
  • 通讯作者: 方雪恩方雪恩 E-mail:fxech@fudan.edu.cn
  • 基金资助:
    国家自然科学基金(82561128250, 2025DNJP0208, 22374029)；国家“万人计划”青年拔尖人才项目(QWH1615018)；上海市科技创新行动计划(23ZR1403200)；复旦大学本科教学内涵提升计划及本科教学研究与改革实践项目(FD2023A223126)；普化实验上海市级重点课程项目

Theory and practice teaching exploration of microfluidic analysis in chemistry “101 Plan”

Lin Liu1, Min Gu1, Zhongyi Yan1, Huiwen Xiong2, Xueen Fang1   

  1. 1 Department of Chemistry, Fudan University, Shanghai 200433, China;
    2 Shanghai Children's Hospital, Department of Laboratory Medicine, Children's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Received:2025-11-26 Accepted:2026-02-06 Published:2026-06-18

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摘要： 面向化学“101计划”的核心教案模块，以“理论-实践”一体化的模式进行了微流控分析教学探索，包含微流控理论教学和微流控核心实验教学两大模块，其中实验教学包含两个案例：转基因大豆核酸检测(LAMP核酸检测案例)和降钙素原快速定量检测(夹心型免疫分析案例)。本课程在传统分析化学教学模式的基础上进行创新，丰富了微流控实验中即时检测(POCT)的理论及实验的课程设计，通过实际案例帮助学生更好地理解微流控的基本原理和实际应用，同时培养学生的实验技能、数据分析能力和综合思维能力。

关键词: 化学“101计划”, 分析化学, 微流控, POCT, 转基因大豆, 降钙素原

Abstract: This paper aimed at the core teaching module of the “101 Plan” in chemistry, and explored the teaching of microfluidic analysis with the mode of “theory-practice” integration, including microfluidic theory teaching and microfluidic core experimental teaching. The laboratory teaching includes two cases: transgenic soybean nucleic acid rapid detection (LAMP nucleic acid detection case) and rapid quantitative analysis of procalcitonin (sandwich immunoassay case). This course innovated on the basis of the traditional teaching mode of analytical chemistry, enriched the design of theory and experiments of POCT (point-of-care testing) in microfluidic experiments, helps students better understand the basic principles and practical applications of POCT through practical cases, and cultivates their experimental skills, data analysis ability and comprehensive thinking ability.

Key words: Chemistry “101 Plan”, Analytical chemistry, Microfluidics, POCT, Transgenic soybean, Procalcitonin