Abstract: This experiment aims to analyze halogenated quinone compounds in environmental water samples using dispersive solid-phase extraction (dSPE) combined with liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS). Five types of halogenated quinone compounds were used as analytes, and a cationic covalent organic framework material was employed as the solid-phase adsorbent to concentrate the target compounds from the water samples. Subsequently, the enriched compounds were subjected to qualitative and quantitative analysis by using LC-MS/MS. The experiment systematically examined the impact of various parameters on the experimental results, including the amount of the adsorbent, enrichment time, elution time, types of elution solvent, acidity of the elution solvent, and elution volume. A novel approach based on dSPE was developed. The method exhibited favorable linearity (with a correlation coefficient of R² ≥ 0.9924) in the range of 0.1-100 ng·L^-1, with detection limits ranging from 0.03-0.65 ng·L^-1. The method was successfully applied to the analysis of halogenated quinone compounds in tap water samples. This experiment helps students understand fundamental sample preparation techniques and enhances their practical skills, research capabilities, and problem-solving abilities.

Key words: Dispersive solid-phase extraction, Liquid chromatography coupled-triple quadrupole mass spectrometry, Halogenated quinone compounds, Water