Abstract: The determination of stereochemistry represents a fundamental aspect in both Organic Chemistry and Medicinal Chemistry, constituting an essential competency for graduate students in related disciplines. While the Mosher method has been extensively employed for stereochemical determination of secondary alcohols and amines in pharmaceutical molecules, dedicated pedagogical reports remain scarce. This paper implemented the BOPPPS teaching model, integrating information technology, to develop and execute a graduate-level curriculum focusing on spectral analysis through authentic research cases. The instructional design aimed to facilitate students' rapid acquisition of the Mosher method's theoretical principles, practical applications, and operational techniques. The results demonstrated that this teaching approach not only enhanced students' comprehension of theoretical knowledge but also significantly improved their problem-solving capabilities in practical contexts, thereby presenting a valuable pedagogical model worthy of broader dissemination and adaptation.

Key words: BOPPPS teaching model, Stereochemistry, Mosher method, Spectral analysis