Abstract: The application of self-constructed instruments in experimental teaching presents a new challenge in the reform of instrumental analysis experiment courses. In these experiments, students independently design and construct Raman spectrometers. By replacing optical components of different specifications and adjusting experimental parameters, students systematically explore various factors affecting the technical performance of the instruments. They utilize the self-constructed Raman spectrometers to detect and identify amino acids, mastering the method of using Raman spectroscopy for molecular structural analysis. This experiment aligns with the requirements of the chemistry “101 Plan” course design, emphasizing the practical teaching mode of process participation. While stimulating students' interest in learning, it focuses on cultivating their scientific exploration spirit and strengthening the connection between theory and practice.

Key words: Self-constructed instrument, Raman spectroscopy, Experimental teaching, Instrumental analysis, Amino acid