Abstract: To meet the urgent demand for cultivating interdisciplinary talents in green pesticide innovation, this experimental design adopts a “research-integrated” approach to establish a virtual-physical hybrid teaching model that combines computer-aided screening, organic synthesis, and bioactivity evaluation. The model systematically integrates multiple disciplines including computational chemistry, organic chemistry, and chemical biology, while incorporating authentic research processes throughout the entire teaching workflow. Using succinate dehydrogenase (SDH) as the molecular target, the experiment focuses on the discovery and activity validation of the novel fungicide fenopyramide. The teaching process begins with molecular docking and virtual screening to help students understand target-oriented molecular design principles, followed by compound synthesis and structural characterization to enhance experimental and analytical skills. The final phase involves enzyme inhibition and hyphal growth inhibition assays, completing the full research cycle from molecular recognition to biological function validation. This modular design reflects the scientific logic of “theoretical construction–experimental verification–result analysis”, effectively fostering students’ interdisciplinary thinking, innovative capacity, and practical skills while creating an integrated platform for green pesticide development and experimental education.

Key words: Virtual-physical integration, Green fungicide, Fenopyramide, Succinate dehydrogenase, Bioactivity