大学化学 >> 2023, Vol. 38 >> Issue (1): 187-191.doi: 10.3866/PKU.DXHX202202025

化学实验 上一篇    下一篇

手性毛细管电泳实验中手性选择剂取代度的影响研究

谢天尧(), 邓爱玲, 肖华, 朱芳   

  • 收稿日期:2022-02-12 录用日期:2022-03-24 发布日期:2022-04-08
  • 通讯作者: 谢天尧 E-mail:cesxty@mail.sysu.edu.cn
  • 基金资助:
    中山大学实验教学研究(改革)项目

Study on the Influence of the Substitution Degree of Hydroxypropyl-β-Cyclodextrin in Chiral Capillary Electrophoresis Experiments

Tianyao Xie(), Ailing Deng, Hua Xiao, Fang Zhu   

  • Received:2022-02-12 Accepted:2022-03-24 Published:2022-04-08
  • Contact: Tianyao Xie E-mail:cesxty@mail.sysu.edu.cn

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摘要：

针对手性毛细管电泳实验“手性药物氧氟沙星对映体的分离检测”的教学过程中，发现手性选择剂取代度对实验结果具有重要影响，详细研究了一系列不同取代度的羟丙基-β-环糊精对氧氟沙星对映体分离效果的影响。研究结果表明：对映体的分离度随取代度变化显著，且取代度DS = 7.9时分离度最佳。依据取代度的测定方法，对市售羟丙基-β-环糊精的质量进行评估，以确保手性毛细管电泳实验结果的稳定性和良好的教学效果。

关键词: 手性毛细管电泳实验, 取代度, 手性药物分析, 羟丙基-β-环糊精, 氧氟沙星, 仪器分析实验

Abstract:

Because of the important influence of the chiral selector substitution degree on the enantioseparation of ofloxacin in chiral capillary electrophoresis experiments, the effects of the substitution degree of hydroxypropyl-β-cyclodextrin (HP-β-CD) on the inclusion complex between ofloxacin and HP-β-CD were investigated according to different substitution degrees. The results showed that the enantioseparation resolution changed significantly with the substitution degree. Moreover, the best separation resolution was found for a DS (degree of substitution) = 7.9. The methods of determining DS were also discussed. Using these methods, the quality of commercial hydroxypropyl-β-cyclodextrin products can be effectively monitored, and the stability and effectiveness in teaching of the chiral capillary electrophoresis experiment can be ensured.

Key words: Chiral capillary electrophoresis experiment, Substitution degree, Chiral drugs analysis, Hydroxypropyl-β-cyclodextrin, Ofloxacin, Instrumental analysis experiment