大学化学 >> 2024, Vol. 39 >> Issue (4): 87-93.doi: 10.3866/PKU.DXHX202310120

所属专题： 化学实验教学和管理(2023)

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仪器分析化学实验：降钙素原快速定量微流控芯片检测

辜敏1, 熊桧文1, 刘丽玲2, 孔继烈1, 方雪恩1   

  1. 1 复旦大学化学系, 上海 200433;
    2 上海速芯生物科技有限公司, 上海 201321
  • 收稿日期:2023-10-31 修回日期:2023-12-05 发布日期:2023-12-20
  • 通讯作者: 方雪恩 E-mail:fxech@fudan.edu.cn
  • 基金资助:
    教育部基础学科拔尖学生培养计划2.0(20222064)；复旦大学本科教学内涵提升计划及本科教学研究与改革实践项目(FD2023A223126)；普化实验上海市级重点课程项目

Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment

Min Gu1, Huiwen Xiong1, Liling Liu2, Jilie Kong1, Xueen Fang1   

  1. 1 Department of Chemistry, Fudan University, Shanghai 200433, China;
    2 Shanghai Suxin Biotechnology Co. Ltd., Shanghai 201321, China
  • Received:2023-10-31 Revised:2023-12-05 Published:2023-12-20

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摘要： 本文设计了一个基于微流控芯片技术检测降钙素原含量的蛋白免疫分析实验，通过溶液的配制、微流控芯片的制备、标准曲线的绘制和未知液样本测试等实验过程，让学生了解微流控芯片技术这一新兴科学研究领域，增强学生的动手能力，锻炼学生的科研思维，在培养化学研究兴趣的同时提升创新思维和知识融会贯通的能力。

关键词: 微流控芯片, 降钙素原, 仪器分析化学实验

Abstract: In this paper, we designed a protein immunoassay using microfluidic technology to quantitatively detect procalcitonin (PCT). The experiment involved various steps such as solution preparation, microfluidic chip assembly, standard curve drawing, testing of unknown liquid samples, and other experimental processes. By conducting these experiments, students can gain a deeper understanding of the emerging scientific research field of microfluidics. Furthermore, this training helps to foster their scientific thinking, ability to innovate, and integrate knowledge.

Key words: Microfluidic chip, Procalcitonin (PCT), Instrumental analytical chemistry experiment