大学化学 >> 2025, Vol. 40 >> Issue (10): 243-249.doi: 10.12461/PKU.DXHX202412043

化学实验 上一篇    下一篇

多种气相色谱定量分析方法同步应用于中药制剂中有效成分的测定

彭淑女, 李华敏, 陈招斌, 王翊如   

  1. 厦门大学化学化工学院, 化学国家级实验教学示范中心(厦门大学), 福建厦门 361005
  • 收稿日期:2024-12-04 录用日期:2025-01-22 发布日期:2025-09-17
  • 通讯作者: 王翊如 E-mail:yrwang@xmu.edu.cn
  • 基金资助:
    教育部基础学科拔尖学生培养计划2.0 (20222111, 20232023);福建省本科高校教育教学研究重大项目(FBJY20230270);国家自然科学基金(32230063)

Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations

Shunü Peng, Huamin Li, Zhaobin Chen, Yiru Wang   

  1. National Demonstration Center for Experimental Chemistry Education (Xiamen University), College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China
  • Received:2024-12-04 Accepted:2025-01-22 Published:2025-09-17
  • Contact: Yiru Wang E-mail:yrwang@xmu.edu.cn

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摘要： 本文改进了一个用气相色谱法分离分析中药制剂中五种主要成分桉叶油醇、樟脑、薄荷醇、水杨酸甲酯、丁香酚含量的本科仪器分析实验。提出同时用内标法、外标法、归一化法对同一组数据文件进行定量并判断定量结果的准确性，使学生能在有限的教学时间内掌握色谱基础的定性定量方法，了解不同定量分析方法的适用范围，学会计算理论塔板数及分离度等。本实验将基本的色谱理论知识融入生活用品风油精等中药制剂的主成分分析中，使学生真切感受学以致用，且绿色环保，有利于培养学生分析实验数据的能力。

关键词: 气相色谱, 风油精, 仪器分析实验, 定性定量方法

Abstract: This study enhances an undergraduate instrumental analysis experiment focused on the separation and quantification of five principal components—eucalyptol, camphor, menthol, methyl salicylate, and eugenol—in traditional Chinese medicine preparations using gas chromatography. It introduces a simultaneous application of internal standard, external standard, and normalization methods to the same dataset to assess the accuracy of quantitative results. This approach enables students to grasp fundamental chromatographic qualitative and quantitative techniques, understand the applicability of various quantitative methods, and learn to calculate theoretical plate numbers and resolution within a limited teaching timeframe. By integrating basic chromatographic theory with the analysis of main components in everyday items such as wind oil essence, this experiment not only demonstrates the practical application of theoretical knowledge but also promotes environmental sustainability. It effectively cultivates students' capabilities in analyzing experimental data.

Key words: Gas chromatography, Wind oil essence, Instrumental analysis experiment, Qualitative and quantitative methods