大学化学 >> 2026, Vol. 41 >> Issue (8): 389-401.doi: 10.12461/PKU.DXHX202507135

化学实验 上一篇    下一篇

一种新型荧光指示剂应用于配位滴定法中铜含量的测定——分析化学实验教学设计与实践

刘秀红, 闫村旭, 张悦, 高雪松, 何海峰   

  1. 江西科技师范大学化学化工学院, 江西 南昌 330038
  • 收稿日期:2025-07-28 录用日期:2025-10-17 发布日期:2026-08-25
  • 通讯作者: 刘秀红, 何海峰 E-mail:xiuhong0627@163.com;hehf0427@jxstnu.com.cn Xiuhong Liu, Haifeng He
  • 基金资助:
    2024年江西省普通本科高校教育教学研究改革课题(JXJG-24-10-12)；2024年江西省教育科学“十四五”规划职业教育核心课程资源研究专项课题(24ZJZX007)；2023年江西省学位与研究生教育教学研究改革课题(JXYJG-2023-144)；2023年江西科技师范大学校级教学改革研究课题(XJJG-2023-89-47)

Coordination titration for copper ion determination using a novel fluorescent turn-on indicator: design and implementation of an analytical chemistry experiment

Xiuhong Liu, Cunxu Yan, Yue Zhang, Xuesong Gao, Haifeng He   

  1. School of Chemistry and Chemical Engineering, Jiangxi Science and Technology Normal University, Nanchang 330038, Jiangxi Province, China
  • Received:2025-07-28 Accepted:2025-10-17 Published:2026-08-25
  • Contact: Xiuhong Liu, Haifeng He E-mail:xiuhong0627@163.com;hehf0427@jxstnu.com.cn

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摘要： 基于“以学生为中心”的教学理念引导学生通过理论与实践结合、实验设计、验证实施，将一种新型荧光化合物作为配位滴定分析实验中的荧光指示剂应用于铜含量的分析，加深了学生对配位滴定分析原理的理解，培养了学生的探究意识，提升了学生的创新意识。与传统的颜色指示剂不同，本实验中使用的荧光指示剂通过滴定终点时从“关”到“开”的荧光突变现象来指示终点，其对Cu2+的高灵敏度响应可以实现对含Cu2+浓度低至1 × 10-5 mol·L-1的定量分析，且无需借助昂贵的荧光分光光度计，滴定分析操作简单，实验过程绿色安全，准确度高，学生参与实验的兴趣浓厚、成就感高。

关键词: 荧光指示剂, 配位滴定, 铜离子, 低浓度, 实验教学

Abstract: Guided by the student-centered teaching philosophy, it combines theoretical knowledge with practical application, experimental design with verification procedures, enabling students to employ a novel fluorescent compound as an indicator in coordination titration for copper content determination. The experiment enhances students' comprehension of coordination titration principles while fostering their investigative skills and innovative thinking. Distinct from conventional colorimetric indicators, the fluorescent indicator used in this study demonstrates a unique “off-on” fluorescence transition at the endpoint, the high sensitivity response of this new fluorescent indicator to Cu2+ can achieve quantitative analysis of Cu2+ concentration as low as 1 × 10-5 mol·L-1, and eliminating the need for expensive fluorescence spectrophotometers. The titration procedure is straightforward, environmentally benign, and safe, with high analytical accuracy. Students demonstrated strong engagement and achieved considerable satisfaction through this experimental learning experience.

Key words: Fluorescent indicator, Coordination titration, Copper ion, Low concentration, Experiment teaching