University Chemistry ›› 2021, Vol. 36 ›› Issue (9): 2101012.doi: 10.3866/PKU.DXHX202101012

Special Issue:

• Chemistry Laboratory • Previous Articles     Next Articles

An Experiment for Determining Copper(Ⅱ) Ions in Copper-Containing Sewage by UV-Vis Spectrophotometry

Ziheng Zhang, Ran Hu, Yijin Ma, Xingrong Sun, Siwei Ma, Ren Zhu, Qiue Cao, Chuanhua Zhou()   

  • Received:2021-01-07 Accepted:2021-01-27 Published:2021-02-22
  • Contact: Chuanhua Zhou


A teaching experiment for determining copper(Ⅱ) ions in copper-containing sewage by UV-Vis spectrophotometry is designed. The experiment is performed based on the Fenton reaction principle, where the presence of copper(Ⅱ) ions in the assay solution catalyzes the decomposition of hydrogen peroxide to produce hydroxyl radicals. The latter oxidizes the colorless 3, 3', 5, 5'-tetramethylbenzidine (TMB) in the solution to the yellow TMB2+, which can be used for quantitative analysis copper(Ⅱ) ions in copper-containing sewage by measuring the absorbance of TMB2+ at 452 nm. With easy operation, short measurement time, excellent stability, eco-friendliness, this experiment conforms to the basic requirements of undergraduate laboratory teaching.

Key words: Fenton reaction, Cu(Ⅱ) detection, Ultraviolet-visible spectrophotometry, Undergraduate teaching experiment