Abstract:

An exploratory analytical chemistry comprehensive experiment was designed based on Prussian blue analog-derived nanoenzymes. The experiment includes the preparation and characterization of three kinds of Prussian blue analog-derived nanoenzyme materials and the determination of their enzyme catalytic reaction rate constants. These nanomaterials were further applied to hydrogen peroxide detection. As this experiment features inexpensive reagents, mild reaction conditions, obvious experimental phenomena, and reasonable time allocation, it is suitable for undergraduate laboratory teaching to improve students' experimental skills and cultivate their scientific research and innovation ability.

Key words: Prussian blue nanomaterials, Enzyme catalytic reaction kinetics, Michaelis constant, H₂O₂ detection, UV-Vis spectrophotometry