Abstract: This study introduces an advanced research protocol from current literature—employing nano covalent organic framework (COF) materials to regulate intracellular Ca²⁺ concentration for enhanced photodynamic therapy (PDT)—as a scalable, multi-level comprehensive chemistry experiment for undergraduate education. We developed a digital virtual simulation platform for experimental teaching and implemented it in undergraduate chemistry curricula. The platform incorporates mathematical, physical, and database models of the experimental system, utilizing digital technology to virtually simulate experimental conditions, thereby achieving high-fidelity replication of experimental procedures and visual presentation of results. Compared with traditional laboratory experiments, the digital platform features modular design that enables students to independently investigate the effects of experimental variables, deepening their subject knowledge while avoiding limitations such as excessive time requirements, high costs, and safety concerns. The teaching process consistently adheres to the student-centered Outcome-Based Education (OBE) approach. Teaching practice demonstrates that this experiment improves students’ instrumental operation skills, data analysis capabilities, and critical thinking, while effectively stimulating learning motivation and creativity. This approach offers new perspectives for optimizing experimental resource allocation and advancing chemistry laboratory education reform.

Key words: Covalent organic framework (COF), Digital experiment, Nanomaterials