Abstract: To address the limitations of conventional chemistry experiment teaching, particularly the lack of frontier scientific integration and low comprehensiveness in experimental content, this study designed an innovative comprehensive experiment focusing on the microfluidic synthesis of lanthanide Eu-metal-organic frameworks (Eu-MOFs) and their fluorescence sensing properties. The experiment employed advanced microfluidic technology for the controlled preparation of Eu-MOFs microspheres, followed by systematic characterization using X-ray diffraction (XRD), inverted microscopy, and fluorescence spectroscopy to investigate their Fe³⁺ sensing capabilities. Comparative results demonstrated that the microfluidically synthesized Eu-MOFs exhibited superior morphological homogeneity and lower detection limits than those prepared by traditional methods, indicating promising potential for metal ion sensing applications. This experiment established a complete training system encompassing “synthesis-characterization-analysis-application” processes, incorporating advanced instrumentation such as fluorescence spectrometers and inverted microscopes. The approach not only enhances students’ mastery of microfluidic technology but also deepens their understanding of coordination chemistry principles and fluorescence sensing mechanisms. This work provides valuable insights for reforming chemical experiment pedagogy and cultivating innovative talents.

Key words: Microfluidic technology, Eu-metal-organic frameworks, Fluorescence quenching, Laboratory education, Comprehensive experiment innovation