Abstract: The existing instrument analysis experiment, “Molecular fluorescence method for the determination of rhodamine B”, is overly simplistic and dose not account for real-world complexities. To address these issues, this improved experiment utilizes three-dimensional fluorescence scanning to acquire sample data. Instead of complex preprocessing steps, chemometric algorithms are applied to extract the pure signals of the target analytes, enabling the simultaneous determination of rhodamine 6G and rhodamine 123 in dyed chili. This improvement enhances students' ability to approach problems comprehensively and develop innovative solutions.

Key words: Rhodamine 6G, Rhodamine 123, Three-dimensional fluorescence method, Chemometrics, Dyed chili