Abstract: This study employed Suzuki coupling reaction to synthesize potassium 5-(4-(diphenylamino)phenyl)thiophene-2-carboxylate (TTC) from 4-triphenylamineboronic acid and 5-bromo-2-carboxythiophene through a series of unit operations including heating, refluxing, filtration, extraction, and drying. The molecular structure and photophysical properties of TTC were systematically characterized using ¹H nuclear magnetic resonance (NMR) spectroscopy, UV-vis absorption spectroscopy, and fluorescence emission spectroscopy. Results demonstrate that the synthesized compound exhibits efficient blue-light emission and remarkable aggregation-induced emission (AIE) characteristics. Building upon these findings, we developed a practical application by designing a latent fingerprint detection protocol. The organic fluorophore serves as an effective fingerprint developer, enabling rapid visualization of high-quality fingerprint images with clear tertiary ridge characteristics. Fingerprint matching was performed using MATLAB-based recognition system. This experiment establishes a complete pedagogical framework encompassing chemical synthesis, material characterization, and application exploration. Preliminary implementation shows excellent experimental reproducibility with visually striking results, effectively stimulating student interest. Through this comprehensive training, students not only acquire interdisciplinary knowledge, but also develop systematic research thinking spanning molecular design, property modulation, and functional applications, while mastering essential techniques in organic synthesis, photophysical characterization, spectral analysis, and fingerprint development.

Key words: Suzuki coupling reaction, Aggregation-induced emission, Latent fingerprint detection, Functional materials