Abstract: This study presents a user-friendly and visually intuitive DNA analysis experiment utilizing rotary microfluidic chip technology, designed to achieve “visible, accurate, and rapid” DNA detection. Using transgenic soybean identification as a model system, we extracted DNA from non-transgenic and two transgenic soybean varieties (BPS-CV127-9 and MON87701), followed by preliminary characterization via UV spectrophotometry and fluorescence analysis. The multicolor fluorescence-based DNA detection method features straightforward procedures and high engagement value, making it suitable for K-12 students and even preschool participants. By employing manual centrifugation and heating plates instead of conventional instruments to drive microfluidic chip reactions, we enabled result visualization through precipitation, pH indicators, and fluorescent markers. This approach is particularly suitable for high school and undergraduate laboratory activities. Furthermore, this experiment serves as an effective platform to debunk pseudoscientific claims about genetically modified crop identification while promoting evidence-based scientific literacy and national regulatory policies. Through tiered outreach activities, we aim to demonstrate chemistry's societal relevance and intrinsic beauty to diverse audiences.

Key words: Microfluidics, DNA, Tiered science outreach, Genetically modified organisms (GMOs), Rapid detection