Abstract: This paper aimed at the core teaching module of the “101 Plan” in chemistry, and explored the teaching of microfluidic analysis with the mode of “theory-practice” integration, including microfluidic theory teaching and microfluidic core experimental teaching. The laboratory teaching includes two cases: transgenic soybean nucleic acid rapid detection (LAMP nucleic acid detection case) and rapid quantitative analysis of procalcitonin (sandwich immunoassay case). This course innovated on the basis of the traditional teaching mode of analytical chemistry, enriched the design of theory and experiments of POCT (point-of-care testing) in microfluidic experiments, helps students better understand the basic principles and practical applications of POCT through practical cases, and cultivates their experimental skills, data analysis ability and comprehensive thinking ability.

Key words: Chemistry “101 Plan”, Analytical chemistry, Microfluidics, POCT, Transgenic soybean, Procalcitonin