大学化学 >> 2019, Vol. 34 >> Issue (5): 46-50.doi: 10.3866/PKU.DXHX201810011

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C18反相色谱固定相的制备及性能评价——介绍一个仪器分析拓展性实验

牛丽红*(),周云,于莹,张源,吴彼伦,麦炜琦   

  • 收稿日期:2018-10-15 录用日期:2018-10-31 发布日期:2018-11-28
  • 通讯作者: 牛丽红 E-mail:niulh@tsinghua.edu.cn
  • 基金资助:
    清华大学教育教学改革基金(53410100537)

Preparation and Performance Evaluation of C18Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment

Lihong NIU*(),Yun ZHOU,Ying YU,Yuan ZHANG,Bilun WU,Weiqi MAI   

  • Received:2018-10-15 Accepted:2018-10-31 Published:2018-11-28
  • Contact: Lihong NIU E-mail:niulh@tsinghua.edu.cn
  • Supported by:
    清华大学教育教学改革基金(53410100537)

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摘要：

介绍了一个本科生仪器分析拓展性实验——C18反相色谱固定相的制备及性能评价。实验内容包含色谱固定相的合成、表征、装填及其性能评价，实验过程涵盖了有机合成、物理化学和仪器分析知识。通过本实验的实践，拓展了经典仪器分析实验内容，有利于培养学生综合运用知识的能力和科研探究能力。

关键词: 仪器分析实验, 色谱, 固定相制备, 性能评价

Abstract:

An extended instrumental analysis experiment "preparation and performance evaluation of C18 stationary phase for reversed-phase chromatography" is recommended for undergraduates. The experiment includes the synthesis, characterization, preparation and properties of the stationary phase. The knowledge of organic synthesis, physical chemistry and instrumental analysis is covered in the experiment. With the practice of the experiment, the typical instrumental analysis experiment is extended. It is helpful for students to cultivate the ability of applying knowledge comprehensively and exercise the ability of scientific research.

Key words: Instrumental analysis experiment, Chromatography, Stationary phase preparation, Performance evaluation