大学化学 >> 2024, Vol. 39 >> Issue (5): 287-295.doi: 10.3866/PKU.DXHX202311096

所属专题: 第4届全国大学生化学实验创新设计大赛

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自制开放式荧光光谱仪的搭建、性能测试与实际应用

孙春霖, 姜姚乐, 陈雨, 郭荣婧, 沈永雯, 惠新平, 张保新, 潘效波   

  1. 兰州大学化学化工学院, 化学国家级实验教学示范中心(兰州大学), 兰州 730000
  • 收稿日期:2023-11-29 修回日期:2024-02-04 发布日期:2024-04-12
  • 通讯作者: 孙春霖,潘效波 E-mail:suncl@lzu.du.cn;boxb@lzu.edu.cn
  • 基金资助:
    兰州大学2023年度教育教学改革研究项目(202317);基础学科拔尖学生培养计划2.0研究课题(20212090);中央高校基本科研业务费(lzujbky-2023-ey03, lzujbky-2017-k07);国家自然科学基金(22075117, 22171111)

Construction, Performance Testing, and Practical Applications of a Home-Made Open Fluorescence Spectrometer

Chun-Lin Sun, Yaole Jiang, Yu Chen, Rongjing Guo, Yongwen Shen, Xinping Hui, Baoxin Zhang, Xiaobo Pan   

  1. National Demonstration Center for Experimental Chemistry Education (Lanzhou University), College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China
  • Received:2023-11-29 Revised:2024-02-04 Published:2024-04-12
  • Contact: Chun-Lin Sun, Xiaobo Pan E-mail:suncl@lzu.du.cn;boxb@lzu.edu.cn

摘要: 荧光检测在化学分析、生物检测等多个领域得到广泛应用,因此对本科生进行相关实验教学具有重要意义。本实验成功创制了一套价格低廉、体积小巧的开放式荧光光谱仪,对其性能进行全面评估,并成功应用于实际样品的检测。这有助于培养学生的仪器设计和创制意识,增强他们对自主创制仪器的信心。

关键词: 荧光检测, 自制荧光光谱仪, 本科实验教学, 四方酸素染料, 国产仪器设备

Abstract: Fluorescence detection has found extensive applications in various fields such as chemical analysis and biological testing. Therefore, conducting experiments related to fluorescence detection is of significant importance in undergraduate laboratory education. This experiment successfully developed a low-cost, compact open-source fluorescence spectrometer, whose performance was comprehensively evaluated and applied to the detection of real samples. The aim is to foster students’ awareness and confidence in designing and creating instruments.

Key words: Fluorescence detection, Home-made fluorescence spectrometer, Undergraduate laboratory teaching, Squaraine dyes, Domestic instruments