大学化学 >> 2025, Vol. 40 >> Issue (5): 351-356.doi: 10.12461/PKU.DXHX202407052

化学实验 上一篇    下一篇

金纳米酶催化过氧化氢分解反应的米氏常数的测定

戴煜, 孙雪婷, 吴昊屿, 李乃竹, 程国娥, 张孝进, 夏帆   

  1. 中国地质大学(武汉)材料与化学学院, 武汉 430078
  • 收稿日期:2024-07-11 修回日期:2024-09-14 发布日期:2025-05-21
  • 通讯作者: 戴煜, 夏帆 E-mail:yudai@cug.edu.cn;xiafan@cug.edu.cn
  • 基金资助:
    湖北本科高校省级教学改革研究项目(2023134)；中国地质大学(武汉)教学改革研究项目(2022006)

Determination of the Michaelis Constant for Gold Nanozyme-Catalyzed Decomposition of Hydrogen Peroxide

Yu Dai, Xueting Sun, Haoyu Wu, Naizhu Li, Guoe Cheng, Xiaojin Zhang, Fan Xia   

  1. Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430078, China
  • Received:2024-07-11 Revised:2024-09-14 Published:2025-05-21
  • Contact: Yu Dai, Fan Xia E-mail:yudai@cug.edu.cn;xiafan@cug.edu.cn

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摘要： 近年来，金纳米粒子被发现具有纳米酶特性，可以模拟天然过氧化物酶催化过氧化氢分解。本实验选用明胶还原制备金纳米粒子，通过紫外-可见分光光度法测定金纳米粒子催化过氧化氢分解反应的米氏常数。将科学前沿概念“纳米酶”设计成适合本科生的物理化学动力学实验，既可锻炼学生的综合能力，又能激发学生对化学实验的兴趣与民族自豪感。

关键词: 纳米酶, 金纳米粒子, 过氧化氢分解, 米氏常数, 物理化学实验

Abstract: In recent years, gold nanoparticles (AuNPs) have been identified as possessing nanozyme properties, enabling them to mimic the catalytic activity of natural peroxidases in the decomposition of hydrogen peroxide (H2O2). In this study, AuNPs were synthesized using gelatin as a reducing agent, and the Michaelis constant for the AuNP-catalyzed decomposition of hydrogen peroxide was determined using UV-Vis spectroscopy. Integrating the innovative concept of “nanozyme” into a physical chemistry kinetics experiment designed for undergraduate students not only enhances their comprehensive skills but also fosters their interest in chemical experimentation and instills a sense of national pride.

Key words: Nanozyme, Gold nanoparticle, Hydrogen peroxide decomposition, Michaelis constant, Physical chemistry experiment