大学化学 >> 2026, Vol. 41 >> Issue (6): 166-174.doi: 10.12461/PKU.DXHX202605044

专题 上一篇    

化学“101计划”综合化学实验教学案例设计——以MOC-16的激光共聚焦细胞成像为例

吴嘉仪1, 王亚平2, 江继军1, 潘梅1, 苏成勇1   

  1. 1 中山大学化学学院, 广东 广州 510006;
    2 中山大学孙逸仙纪念医院, 广东 广州 510030
  • 收稿日期:2026-05-13 录用日期:2026-05-21 发布日期:2026-06-18
  • 通讯作者: 潘梅, 苏成勇 E-mail:panm@mail.sysu.edu.cn;cesscy@mail.sysu.edu.cn Mei Pan, Chengyong Su
  • 基金资助:
    国家自然科学基金项目(92461302)

Case design of the Chemistry “101 Plan” comprehensive chemistry experiment: taking laser confocal cell imaging of MOC-16 as an example

Jiayi Wu1, Yaping Wang2, Jijun Jiang1, Mei Pan1, Chengyong Su1   

  1. 1 School of Chemistry, Sun Yat-sen University, Guangzhou 510006, Guangdong Province, China;
    2 Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510030, Guangdong Province, China
  • Received:2026-05-13 Accepted:2026-05-21 Published:2026-06-18
  • Contact: Mei Pan, Chengyong Su E-mail:panm@mail.sysu.edu.cn;cesscy@mail.sysu.edu.cn

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摘要： 面向化学“101计划”对综合化学实验课程“高阶性、创新性、挑战度”的要求，本文构建了一个以pH响应型有机金属超分子笼MOC-16的生物成像性能为核心、依托大型仪器共享平台的细胞成像综合实验。该实验涵盖了细胞培养、pH依赖性细胞膜靶向孵育、激光共聚焦单/双光子成像及图像分析等环节，形成了完整的科研训练模式，可系统培养学生操作大型仪器的实战能力与跨学科思维。作为化学“强基计划”现代化学研究方法与实验课程的核心模块，本实验已入选化学“101计划”合成化学实验教材综合部分，既可作为化学生物学实验单独开课，也可与超分子笼的合成与表征内容组合作为面向生物医药应用的综合化学实验开设，不仅凝练了契合拔尖创新人才培养目标的实验教学模式，也为大型仪器共享平台支撑基础学科人才培养提供了可复制、可推广的实践范例。

关键词: 化学“101计划”, 综合化学实验, 激光共聚焦显微镜, 大型仪器共享平台, 拔尖人才培养, 超分子材料

Abstract: To meet the requirements of the Chemistry“101 Plan” for a “high-level, innovative, and challenging” comprehensive chemistry experiment course, this paper presents a comprehensive cell imaging experiment based on the biological imaging performance of pH-responsive organometallic supramolecular cage MOC-16 leveraging a large-scale instrument sharing platform. This experiment covers the complete scientific research chain including cell culture, pH-dependent cell membrane targeted incubation, one-/two-photon imaging via confocal laser scanning microscopy and image analysis, which can cultivate students’ practical ability to operate large-scale instruments and interdisciplinary thinking. As the core module of the “Modern Chemical Research Methods and Experiments” course under the chemistry “Strong Foundation Plan”, this experiment has been included in the comprehensive part of the synthetic chemistry experiment textbook of the Chemistry “101 Plan”. It can be offered as a standalone course in chemical biology experiments, or combined with the content of the synthesis and characterization of supramolecular cages to be offered as a comprehensive experiment for biomedical applications. It has refined an experimental teaching model that aligns with the goal of cultivating top-notch innovative talents and has also provided a replicable and scalable model for supporting the cultivation of basic disciplines through a large-scale instrument sharing platform.

Key words: Chemistry “101 Plan”, Comprehensive chemistry experiment, Confocal laser scanning microscopy, Large-scale instrument sharing platform, Top-notch talent cultivation, Supramolecular materials