大学化学 >> 2025, Vol. 40 >> Issue (5): 93-105.doi: 10.12461/PKU.DXHX202408100

所属专题： 化学实验标准与规范建设

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有机化学色谱学实验基本操作规范建议

李厚金1, 吴琳2, 孙兴文3, 郑媛4, 刘占祥5, 蔡双莲6, 熊英7, 余广鳌8, 刘庆文9, 韩杰10, 杜欣11, 袁呈山12, 张奇涵13, 张剑荣2, 张树永14   

  1. 1 中山大学化学学院, 广州 510006;
    2 南京大学化学化工学院, 化学国家级实验教学示范中心(南京大学), 南京 210023;
    3 复旦大学化学系, 化学国家级实验教学示范中心(复旦大学), 上海 200433;
    4 中国科学技术大学化学与材料科学学院, 化学国家级实验教学示范中心(中国科学技术大学), 合肥 230026;
    5 浙江大学化学系, 化学国家级实验教学示范中心(浙江大学), 杭州 310058;
    6 湖南大学化学化工学院, 长沙 410082;
    7 武汉大学化学与分子科学学院, 化学国家级实验教学示范中心(武汉大学), 武汉 430072;
    8 华中师范大学化学学院, 武汉 430079;
    9 吉林大学化学学院, 长春 130012;
    10 南开大学化学学院, 化学国家级实验教学示范中心(南开大学), 天津 300071;
    11 大连理工大学化学学院, 辽宁 大连 116024;
    12 兰州大学化学化工学院, 兰州 730000;
    13 北京大学化学与分子工程学院, 化学国家级实验教学示范中心(北京大学), 北京 100871;
    14 山东大学化学与化工学院, 济南 250100
  • 收稿日期:2024-08-24 修回日期:2024-09-13 发布日期:2025-05-21
  • 通讯作者: 李厚金, 张剑荣, 张树永 E-mail:ceslhj@mail.sysu.edu.cn;jrzhang@nju.edu.cn;syzhang@sdu.edu.cn
  • 基金资助:
    广东省本科高校教学质量与教学改革工程建设项目(化学实验教研室)；广东省高等教育教学改革项目；中山大学本科教学质量工程项目；“教育部化学实验教学改革研究虚拟教研室”项目；教育部2022年基础学科拔尖学生培养计划研究项目(20221008)

Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments

Houjin Li1, Lin Wu2, Xingwen Sun3, Yuan Zheng4, Zhanxiang Liu5, Shuanglian Cai6, Ying Xiong7, Guangao Yu8, Qingwen Liu9, Jie Han10, Xin Du11, Chengshan Yuan12, Qihan Zhang13, Jianrong Zhang2, Shuyong Zhang14   

  1. 1 School of Chemistry, Sun Yat-Sen University, Guangzhou 510006, China;
    2 National Demonstration Center for Experimental Chemistry Education (Nanjing University), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China;
    3 National Demonstration Center for Experimental Chemistry (Fudan University), Department of Chemistry, Fudan University, Shanghai 200433, China;
    4 National Demonstration Center for Experimental Chemistry Education (University of Science and Technology of China), School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230026, China;
    5 National Demonstration Center for Experimental Chemistry Education (Zhejiang University), Department of Chemistry, Zhejiang University, Hangzhou 310058, China;
    6 College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China;
    7 National Demonstration Center for Experimental Chemistry Education (Wuhan University), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;
    8 College of Chemistry, Central China Normal University, Wuhan 430079, China;
    9 National Demonstration Center for Experimental Chemistry Education (Jilin University), College of Chemistry, Jilin University, Changchun 130012, China;
    10 National Demonstration Center for Experimental Chemistry Education (Nankai University), College of Chemistry, Nankai University, Tianjin 300071, China;
    11 School of Chemistry, Dalian University of Technology, Dalian 116024, Liaoning Province, China;
    12 College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;
    13 National Demonstration Center for Experimental Chemistry Education (Peking University), College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;
    14 School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China
  • Received:2024-08-24 Revised:2024-09-13 Published:2025-05-21
  • Contact: Houjin Li, Jianrong Zhang, Shuyong Zhang E-mail:ceslhj@mail.sysu.edu.cn;jrzhang@nju.edu.cn;syzhang@sdu.edu.cn

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摘要： 薄层色谱、柱色谱、纸色谱实验是有机化学实验的基本内容。熟练掌握并运用色谱技术分析、纯化、鉴定有机化合物是高校化学类专业学生必须掌握的基本实验技能。目前各高校色谱学实验的训练内容、要求和标准各不相同，达成的教学效果也存在较大差距。本文针对薄层色谱、柱色谱、纸色谱实验提出了基本操作与规范建议，希望能为国内同行开展实验教学以及科研实践提供参考。

关键词: 有机化学实验, 薄层色谱, 柱色谱, 纸色谱, 操作规范

Abstract: Chromatography experiments are a fundamental experimental skill that must be mastered by the students majoring in chemistry in universities. Thin-layer chromatography, column chromatography, and paper chromatography experiments constitute the basic content of organic chemistry experiments. However, the training content, requirements, and standards for chromatography experiments vary among different universities, and lead to disparities in teaching outcomes. This article puts forward basic operation and standard suggestions for organic chemical layer chromatography, column chromatography and paper chromatography experiments, hoping to provide references for domestic peers in conducting experimental teaching and scientific research practices.

Key words: Organic chemistry experiment, Thin layer chromatography, Column chromatography, Paper chromatography, Operation standard