Abstract:

Humic acid is the major component of natural organic matters, which possesses a complex structure and multiple emission characteristics. Considering the research topics of humic acid or other natural organic matters, humic acid was used for steady-state and time-resolved fluorescence experimental teaching. Students can acquire the knowledge about the Raman scattering and inner filtering effects on fluorescence measurements, the effects of excitation energy on the emission spectrum, and fluorescence lifetime.

Key words: Molecular fluorescence, Fluorescence lifetime, Humic acid, Raman scattering