超高效合相色谱法测定咖啡因含量实验设计

doi:10.3866/PKU.DXHX202011055

Abstract

Abstract:

An experiment of instrumental analysis for determination of caffeine content in beverages by Ultra Performance Convergence Chromatography (UPC²) was designed. The caffeine in three beverages including coke, green tea and oolong tea was extracted and diluted by methanol. Then caffeine concentrations in beverages were measured by UPC². Through this experiment, students could not only consolidate several laboratory skills such as weighing, constant volume and liquid transfer, but also master the basic theory, operation and data processing of UPC². This experiment integrates the new chromatography into basic laboratory teaching, which can broaden students' scientific horizons. It is conductive to cultivating students' innovative spirit and practical ability.

Key words: UPC², Caffeine, Content determination, Laboratory teaching

Yifan Xie, Liyun Yao, Zhonghong Yan, Yuxing Cai, Yujie Jin, Ning Li. Design of Experiment for Determination of Caffeine Content by Ultra Performance Convergence Chromatography[J].University Chemistry, 2021, 36(9): 2011055.

Figures/Tables 7

References 13

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样品	峰面积	浓度/(μg?mL^?1)	饮料中咖啡因浓度/(μg?mL^?1)
标样1	1162	0.56
标样2	1995	1.12
标样3	9563	5.61
标样4	14924	8.97
标样5	18210	11.22
乌龙茶	11979	7.25	144.91
绿茶	8037	4.82	96.46
可乐	7329	4.39	87.76

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Design of Experiment for Determination of Caffeine Content by Ultra Performance Convergence Chromatography

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