邻苯二甲醛对还原型谷胱甘肽的光谱分析——推荐一个仪器分析实验

doi:10.3866/PKU.DXHX202012040

University Chemistry ›› 2021, Vol. 36 ›› Issue (9): 2012040.doi: 10.3866/PKU.DXHX202012040

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Optical Analysis for Reduced Glutathione Using o-Phthalaldehyde: Recommend an Instrumental Analysis Experiment

Peng Zhang, Qian Zhang, Hua Zhong, Caifeng Ding()

Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong Province, China

Received:2020-12-24 Accepted:2021-01-18 Published:2021-01-22
Contact: Caifeng Ding E-mail:dingcaifeng@qust.edu.cn

Abstract

Abstract:

In this paper, an innovative experiment of instrumental analysis was recommended to detect reduced glutathione (GSH) by using commercial reagent o-phthalaldehyde. The experiment involves preparation of standard solutions, spectra analysis, and measurement of ¹H nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry (HR-MS). The implementation of this experiment can help students consolidate the basics knowledge of analytical chemistry and organic chemistry, understand the frontier scientific research, cultivate the scientific research ability and research ideas, and stimulate their enthusiasm for scientific research.

Key words: Small molecule probe, UV-Vis spectrum, Fluorescence spectrum, ¹H NMR, HR-MS

Peng Zhang, Qian Zhang, Hua Zhong, Caifeng Ding. Optical Analysis for Reduced Glutathione Using o-Phthalaldehyde: Recommend an Instrumental Analysis Experiment[J].University Chemistry, 2021, 36(9): 2012040.

Figures/Tables 10

References 12

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序号	[OPA]	[GSH]	X	序号	[OPA]	[GSH]	X
1	1000	0	0	7	400	600	0.6
2	900	100	0.1	8	300	700	0.7
3	800	200	0.2	9	200	800	0.8
4	700	300	0.3	10	100	900	0.9
5	600	400	0.4	11	0	1000	1
6	500	500	0.5

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Optical Analysis for Reduced Glutathione Using o-Phthalaldehyde: Recommend an Instrumental Analysis Experiment

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