University Chemistry ›› 2021, Vol. 36 ›› Issue (9): 2012040.doi: 10.3866/PKU.DXHX202012040

Special Issue:

• Chemistry Laboratory • Previous Articles     Next Articles

Optical Analysis for Reduced Glutathione Using o-Phthalaldehyde: Recommend an Instrumental Analysis Experiment

Peng Zhang, Qian Zhang, Hua Zhong, Caifeng Ding()   

  • Received:2020-12-24 Accepted:2021-01-18 Published:2021-01-22
  • Contact: Caifeng Ding


In this paper, an innovative experiment of instrumental analysis was recommended to detect reduced glutathione (GSH) by using commercial reagent o-phthalaldehyde. The experiment involves preparation of standard solutions, spectra analysis, and measurement of 1H nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry (HR-MS). The implementation of this experiment can help students consolidate the basics knowledge of analytical chemistry and organic chemistry, understand the frontier scientific research, cultivate the scientific research ability and research ideas, and stimulate their enthusiasm for scientific research.

Key words: Small molecule probe, UV-Vis spectrum, Fluorescence spectrum, 1H NMR, HR-MS