Abstract:

The paper designed a chemical experiment based on carbon dots which own excellent fluorescence properties. Students need to synthesize blue and green carbon dots through "bottom-up" method, search the relationship between fluorescence excitation spectra and fluorescence emission spectra, measure the fluorescence quantum yield by comparing with a reference fluorophore, explore the changes of fluorescence spectra at different concentrations and pH, and apply the carbon dots to anti-counterfeit materials. All these will make students have a good command of fluorescence principle, such as fluorescence excitation spectrum, fluorescence emission spectrum, fluorescence quantum yield, Stokes shift and the factors that influence fluorescence. We also encourage students to apply the knowledge they have learned to their daily life.

Key words: Fluorescent carbon dots, Fluorescence quantum yield, Stokes shift, Fluorescence spectrum, Fluorescence ink