University Chemistry ›› 2015, Vol. 30 ›› Issue (5): 39-44.doi: 10.3866/pku.DXHX20150539

• Chemistry Laboratory • Previous Articles     Next Articles

Optimization for Determination of PolypeptidesRelative Molecular Mass by MALDI-TOF MS

Yu Huijuan1, Chu Yuehuan1, Shao Guang1, Jiang Fan2, Chao Hui1   

  1. 1. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, Guangdong, China;
    2. The Testing and Technology Center for Industrial Products, Shenzhen Enter-exit Inspection and Quarantine Bureau, Shenzhen 518017, Guangdong, China
  • Published:2015-10-24


In this paper, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI- TOF MS) was used for measuring the relative molecular mass of polypeptides, and the matrixes, solvents, and adding cationic reagents were investigated for optimized determinations.

Key words: Matrix-assisted laser desorption/ionization time of flight mass spectrometry, Polypeptides, Deter- mination of relative molecular mass