Abstract: Two protocols, the transient method and the mathematical method, are introduced to deduce all the rate constants in the enzyme-catalyzed reaction model based on the Michaelis-Menten mechanism. The transient method focuses on analyzing the period before reaching a steady state, while the mathematical method aims to establish a relationship between the concentration of the enzyme and the substrate. By calculating all the rate constants, one can enhance their understanding and grasp of the kinetics of the enzyme-catalyzed reaction.

Key words: Enzyme catalyzed reaction, Kinetics, Michaelis-Menten mechanism, Rate constant