Abstract: This study integrates organic and analytical chemistry to develop a fluorescence detection experiment for Al³⁺, featuring a hydrazone-based probe (Pro-MZ) designed through a photo-induced electron transfer (PET) mechanism. Pro-MZ specifically binds Al³⁺ at pH 7.4, achieving a 30-fold fluorescence enhancement at 471 nm with a detection limit of 38.0 nmol∙L^-1 (3σ/S) and superior selectivity. Practical sample detection demonstrated recoveries of 98.7%–103.2%. The 10-period interdisciplinary curriculum combines molecular synthesis, spectral analysis, and real-world application validation, enhancing students’ research and innovative capabilities while establishing a novel pedagogical framework for fluorescence probe education.

Key words: Fluorescent probe, Al³⁺ detection, PET mechanism, Comprehensive chemistry experiment, Pedagogical innovation