DNA历险记——氧化损伤篇

doi:10.3866/PKU.DXHX202204087

Abstract

Abstract:

DNA damage is a change in DNA structure caused by external or internal factors, including DNA alkylation, oxidation, mismatch, breakage. Although the DNA damage repair mechanism can repair damaged DNA to a certain extent, accumulated damage that is not repaired can seriously threaten genome stability and human life. Oxidative DNA damage is one of the most common types of damage, with 8-hydroxydeoxyguanosine as its biomarker. The quantitative detection of 8-hydroxydeoxyguanosine can reflect the degree of oxidative DNA damage, which is very important for the early diagnosis and treatment of diseases. In this paper, we introduce the production process, repair mechanism, and detection methods for 8-hydroxydeoxyguanosine.

Key words: DNA damage and repair, Oxidative DNA damage, 8-hydroxydeoxyguanosine, Production process, Repair mechanism, Detection methods

Zhe Guan, Hongjie Song, Rui Liu. Adventures of DNA: Oxidative Damage[J].University Chemistry, 2022, 37(9): 2204087.

Figures/Tables 7

References 22

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分析方法	检出限	线性范围	线性相关系数	平均加标回收率	参考文献
HPLC-ECD	13 pg∙mL⁻¹	0.01–15 ng∙mL⁻¹	0.989–0.999	96.52%	20
GC-MS	0.14 ng∙mL⁻¹	1.4–2800 ng∙mL⁻¹	0.9989	99.37%	21
ELISA	0.5 ng∙mL⁻¹	0.5–60 ng∙mL⁻¹	0.9	–	22
PY共振光散射	15.2 ng∙mL⁻¹	50–226.4 ng∙mL⁻¹	0.999	92.2%	18
³²P后标记法	1个8-OHdG/10⁶核苷	–	–	–	17

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Adventures of DNA: Oxidative Damage

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