University Chemistry ›› 2023, Vol. 38 ›› Issue (9): 122-130.doi: 10.3866/PKU.DXHX202210053

• Chemistry Laboratory • Previous Articles     Next Articles

Synthesis and Characterization of DNA-Mediated Fluorescent Copper Nanoclusters: Recommending A Research-Oriented Comprehensive Chemical Experiment

Junbin Li, Chenglin Li, Jiaqi Luo, Hui Chen, Zhihe Qing()   

  • Received:2022-10-20 Accepted:2022-12-09 Published:2023-09-21
  • Contact: Zhihe Qing E-mail:qingzhihe@hnu.edu.cn

Abstract:

To achieve the goals of scientific research on back-feeding teaching and teaching innovation to promote personnel training, reforming traditional chemical experiments is necessary. Based on previous scientific research achievements of the research team, a comprehensive chemical experiment was designed to synthesize and characterize fluorescent copper nanoclusters using DNA molecules as templates, and the conditions for stable synthesis of fluorescent copper nanoclusters were obtained. The experiment included the synthesis of nanoclusters, use of basic spectral analysis instruments, and spectral data processing. The experiment integrates the frontier knowledge of analytical chemistry, biochemistry, and nanoscience, among others. The experimental conditions were simple and mild, and the experimental duration was reasonable, meeting the requirements of experimental teaching, stimulating students' interest in scientific research, enhancing their comprehensive ability, and promoting experimental teaching.

Key words: DNA, Copper nanoclusters, Fluorescence