University Chemistry ›› 2023, Vol. 38 ›› Issue (9): 196-201.doi: 10.3866/PKU.DXHX202211056

• Chemistry Laboratory • Previous Articles     Next Articles

Experimental Design for HPLC Determination of Vitamin B2 in Tablets via UVD-FLD Connected in Series

Yifan Xie1,2, Liyun Yao1,2,*(), Ruolin Yang2, Yuxing Cai1, Yujie Jin1, Ning Li1   

  1. 1 Basic Medical Experimental Teaching Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
    2 Medical Chemistry and Bioinformatics Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
  • Received:2022-11-23 Accepted:2023-01-03 Published:2023-09-21
  • Contact: Liyun Yao E-mail:yaoliyun517@163.com

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Abstract:

The high performance liquid chromatography (HPLC) method of UV detector-fluorescence detector (UVD-FLD) connected in series for the determination of vitamin B2 in tablets was designed as a teaching experiment. This method can overcome the limitations of common HPLC by a single detector. Through the application of this method for experimental teaching, students could not only master the basic theory and operation of HPLC, but also understand the principles and functions of UVD and FLD. Furthermore, it also improved the undergraduate students' experimental skill and comprehension.

Key words: HPLC, UV detector-fluorescence detector, Vitamin B2, Content determination, Laboratory teaching