Abstract: This experiment addresses the pressing issue of milk safety, integrating cutting-edge molecular imprinting technology into undergraduate laboratory classes. A comprehensive experiment is designed, combining molecular imprinting, protein exclusion, and magnetic separation techniques to synthesize lightweight magnetic molecularly imprinted polymers. The polymers’ performance is evaluated, and they are utilized as solid-phase extraction adsorbents in conjunction with high-performance liquid chromatography to establish a novel method for the direct and selective detection of trace tetracycline in milk samples. This interdisciplinary study spans materials chemistry, analytical chemistry, instrumental chemistry, and food chemistry, fostering students’ scientific literacy, problem-solving abilities, and innovative thinking. The innovative design of the imprinting material and magnetic separation process ensures cost-effectiveness, ease of operation, and high safety throughout the experiment. Through this hands-on experience, students gain insight into molecular imprinting technology, learn new detection methods, refine basic laboratory skills, enhance data collection and analysis capabilities, and deepen their understanding of the interplay between components, performance, and application.

Key words: Molecularly imprinted polymer, Milk, Tetracycline, Comprehensive chemistry experiment