新冠病毒光热/比色双模态定量综合性实验设

doi:10.12461/PKU.DXHX202402027

Abstract

Abstract: We have designed a comprehensive experiment for the photothermal/colorimetric dual-mode quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). By developing label nanoprobes with high photothermal conversion efficiency under near-infrared light and integrating them with a self-built small photothermal imager, we have effectively addressed the limitations of commercial colloidal gold test strips, such as low sensitivity and inability to quantify. This method is rapid and user-friendly, requiring only 15 minutes to complete, and enables both naked-eye colorimetric qualification and photothermal accurate quantification. With a detection limit as low as 1.2 pg·mL^-1, this method surpasses commercial colloidal gold test strips by achieving accurate quantitative detection and improving sensitivity by nearly 10,000 times, significantly reducing the detection window period. Teaching practices have shown that this experiment enhances students’ comprehensive abilities, expands their academic horizons, and nurtures their scientific research literacy and innovation potential.

Key words: Au_shell-Fe₃O₄ nanoparticles, Colorimetric detection, Photothermal detection, Test strip, SARS-CoV-2

Jingbin Zeng, Wenjing Wang, Tianyu Zhao, Gengchen Guo, Chunpeng Jiao, Yongzhong Jiang, Congying Wen. Design of a Comprehensive Experiment for Photothermal/Colorimetric Dual-Mode Quantification of SARS-CoV-2[J].University Chemistry, 2025, 40(2): 1-10.

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Design of a Comprehensive Experiment for Photothermal/Colorimetric Dual-Mode Quantification of SARS-CoV-2

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