Abstract: Single-particle tracking represents a significant technological breakthrough for real-time observation of dynamic biological processes, particularly in uncovering mechanisms hidden in static observations. With advancements in optical microscopy, commercial instruments now enable real-time observation of biological processes at the microscopic scale. This experiment explores the size effect of plasmonic nanomaterials using gold and silver nanoparticles as imaging probes. Additionally, live cell imaging experiments are designed to introduce single-particle tracking into undergraduate education by observing the diffusion of gold nanorods on cell membranes as an exemplary experiment in Chemical Metrology and Technology. The experiment is rich in content and engaging, targeting second- and third-year undergraduates who have a foundational understanding of chemistry and chemical biology and are interested in Chemical Metrology and Technology. The goal is to integrate cutting-edge research in single-particle tracking into undergraduate teaching, encouraging independent exploration and stimulating students’ interest in scientific research while cultivating their professional skills.

Key words: Single-particle tracking, Plasmonic nanoparticles, Live-cell imaging, Integration of science and education, Integrated chemistry experiments