Abstract: High-performance liquid chromatography (HPLC) is extensively utilized across various domains including life science research, clinical medical testing, drug development, and forensic identification. Improving the instructional effectiveness of HPLC instrument operation is crucial for nurturing highly competent and skilled medical technology professionals. However, challenges such as a high student-to-instrument ratio, limited availability of equipment, and the inability to conduct repeated operations have adversely impacted learning outcomes. To address these issues, this study integrates a blended online and offline teaching approach into the HPLC experimental curriculum by leveraging platforms such as Chaoxing Learning and virtual simulation experiments. This method optimizes teaching content across pre-class, in-class, and post-class phases. By employing task-driven strategies, facilitating online self-learning, conducting offline group practical exercises, and encouraging post-class summaries and knowledge expansion, the approach aims to comprehensively enhance student engagement and initiative. Empirical results demonstrate that this teaching model significantly improves instructional effectiveness and is favorably received by students.

Key words: High-performance liquid chromatography, Online and offline hybrid teaching, Chaoxing learning platform, Virtual simulation experiment, Task-driven approach