Abstract: Owing to its distinctive electronic effects, conformational properties, and physicochemical characteristics, fluorine plays a pivotal role in various life science research fields including medicinal chemistry, chemical biology, and medical imaging. However, current undergraduate Organic Chemistry curricula generally lack systematic coverage of organic fluorine chemistry, necessitating significant additional time investment by students to acquire this knowledge during their research careers. This knowledge gap hinders the development of interdisciplinary research competencies. This article proposes an instructional framework centered on six core themes: “fluorine modulation in drug design”, “fluorinated probe molecules”, “fluorination methodologies”, “molecular modeling”, “teaching implementation strategies”, and “teaching evaluation”. The framework aims to help students master the structural principles and design logic of fluorinated molecules, thereby enhancing their capabilities in molecular design, structural analysis, and interdisciplinary practice. This approach provides innovative pedagogical insights for cultivating interdisciplinary talents who meet the demands of modern pharmaceutical research and development.

Key words: Organic fluorine chemistry, Drug design, PET imaging, Trifluoromethylation, Fluorine modulation, Teaching reform