University Chemistry ›› 2017, Vol. 32 ›› Issue (1): 58-61.doi: 10.3866/PKU.DXHX201609031

• Chemistry Laboratory • Previous Articles     Next Articles

Measurement of Tracing Copper Using Catalytic Kinetic Fluorescence Method: Design and Practice of an Inquiry Experiment

Li SHENG*(),Zhi-Hui ZHAO,Ying MI,Bi-Quan SU,Wang-Li JING,Cai-Hong TAO   

  • Published:2017-01-13
  • Contact: Li SHENG
  • Supported by:


Based on the catalytic effects of Cu(Ⅱ) on KIO4 oxidized safranineT, a newsystemfor determining tracing copper with catalytic kinetic fluorescencemethod is established.After adding Cu(Ⅱ), the fluorescence intensity at 580 nmis significantly reduced.Within the concentration range of 2.50-32.50 ng·mL-1, a linear relationship between the concentration of Cu(Ⅱ) and lg(F0/F) in the system is observed. Based on this new experiment, the design and implementation of instrumental analysis laboratory is investigated. Meanwhile, in order to achieve high quality of the inquiry experiment teaching, teachers should be responsible for proposition and feasibility.

Key words: Instrumental analysis laboratory, Design of inquiry experiment, Copper, Catalytic kinetic fluorescence method